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Bethyl sharp
Sharp, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+sharp/SHARP+Antibody/us11828684-570-21-22
Average 91 stars, based on 14 article reviews
sharp - by Bioz Stars, 2026-09
91/100 stars

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Related Articles

Immunoprecipitation:

Article Title: Reactivation of x chromosome genes
Article Snippet: Eluted RNA was purified using the RNA Clean and Concentrator-5 Kit (Zymo Research) and RT-qPCR was performed as described previously (17) to evaluate RNA enrichment. .. The antibodies used for immunoprecipitation were anti-FLAG® M2 (Sigma-Aldrich; F1804) (for ΔTM- and ΔRS-LBR transfected cells), anti-SHARP (Bethyl; A301-119A), and customized LBR antibody from GenScript (LBR #4; 540774-1). ..

Article Title: HDAC inhibitor compositions for reactivation of the X chromosome
Article Snippet: Eluted RNA was purified using the RNA Clean and Concentrator-5 Kit (Zymo Research) and RT-qPCR was performed as described previously (17) to evaluate RNA enrichment. .. The antibodies used for immunoprecipitation were anti-FLAG® M2 (Sigma-Aldrich; F1804) (for ΔTM- and ΔRS-LBR transfected cells), anti-SHARP (Bethyl; A301-119A), and customized LBR antibody from GenScript (LBR #4; 540774-1). ..

Transfection:

Article Title: Reactivation of x chromosome genes
Article Snippet: Eluted RNA was purified using the RNA Clean and Concentrator-5 Kit (Zymo Research) and RT-qPCR was performed as described previously (17) to evaluate RNA enrichment. .. The antibodies used for immunoprecipitation were anti-FLAG® M2 (Sigma-Aldrich; F1804) (for ΔTM- and ΔRS-LBR transfected cells), anti-SHARP (Bethyl; A301-119A), and customized LBR antibody from GenScript (LBR #4; 540774-1). ..

Article Title: HDAC inhibitor compositions for reactivation of the X chromosome
Article Snippet: Eluted RNA was purified using the RNA Clean and Concentrator-5 Kit (Zymo Research) and RT-qPCR was performed as described previously (17) to evaluate RNA enrichment. .. The antibodies used for immunoprecipitation were anti-FLAG® M2 (Sigma-Aldrich; F1804) (for ΔTM- and ΔRS-LBR transfected cells), anti-SHARP (Bethyl; A301-119A), and customized LBR antibody from GenScript (LBR #4; 540774-1). ..

Imaging:

Article Title: On the Role of Three-Dimensional Genome Organization in Gene Regulation and mRNA Splicing
Article Snippet: .. Primary antibodies used in the study: anti-Nucleolin (Abcam Cat# ab22758, RRID:AB_776878, 1:500); anti-NPAT (Abcam Cati# ab70595, RRID:AB_ 1269585, 1:100); anti-SMN (BD Biosciences Cati# 610646, RRID:AB_397973, 1:100); anti-HP18 (Active Motif Cat# 39979, RRID:AB_ 2793416, 1:200); anti-Coilin (Abcam Cat # ab210785; Santa Cruz Biotechnology Cat# sc-55594, RRID:AB_1121780; Santa Cruz Biotechnology Cat# sc-56298, RRID:AB_1121778; 1:100); anti-Sharp (Bethyl Cat# A301-119A, RRID:AB 873132, 1:200); anti-Histone H3K27ac (Active Motif Cat# 39134, RRID:AB_ 2722569); anti-NPM1 (Abcam Cat# ab10530, RRID:AB_ 297271; 1:200); anti-Fibrillarin (Abcam Cat# ab5821, RRID:AB 2105785; 1:200); anti-LaminB1 (Abcam Cat# ab16048, RRID:AB_10107828; 1:1000); For imaging studies, all antibodies were diluted in blocking solution. ..

Article Title: RNA promotes the formation of spatial compartments in the nucleus.
Article Snippet: .. Primary antibodies used in the study: anti-Nucleolin (Abcam Cat# ab22758, RRID:AB_776878, 1:500); anti-NPAT (Abcam Cat# ab70595, RRID:AB_1269585, 1:100); anti-SMN (BD Biosciences Cat# 610646, RRID:AB_397973, 1:100); anti-HP1b (Active Motif Cat# 39979, RRID:AB_2793416, 1:200); anti-Coilin (Abcam Cat# ab210785; Santa Cruz Biotechnology Cat# sc-55594, RRID:AB_1121780; Santa Cruz Biotechnology Cat# sc-56298, RRID:AB_1121778; 1:100); anti-Sharp (Bethyl Cat# A301-119A, RRID:AB_873132, 1:200); anti-Histone H3K27ac (Active Motif Cat# 39134, RRID:AB_2722569); anti-NPM1 (Abcam Cat# ab10530, RRID:AB_297271; 1:200); anti-Fibrillarin (Abcam Cat# ab5821, RRID:AB_2105785; 1:200); anti-LaminB1 (Abcam Cat# ab16048, RRID:AB_10107828; 1:1000); For imaging studies, all antibodies were diluted in blocking solution. ..

Blocking Assay:

Article Title: On the Role of Three-Dimensional Genome Organization in Gene Regulation and mRNA Splicing
Article Snippet: .. Primary antibodies used in the study: anti-Nucleolin (Abcam Cat# ab22758, RRID:AB_776878, 1:500); anti-NPAT (Abcam Cati# ab70595, RRID:AB_ 1269585, 1:100); anti-SMN (BD Biosciences Cati# 610646, RRID:AB_397973, 1:100); anti-HP18 (Active Motif Cat# 39979, RRID:AB_ 2793416, 1:200); anti-Coilin (Abcam Cat # ab210785; Santa Cruz Biotechnology Cat# sc-55594, RRID:AB_1121780; Santa Cruz Biotechnology Cat# sc-56298, RRID:AB_1121778; 1:100); anti-Sharp (Bethyl Cat# A301-119A, RRID:AB 873132, 1:200); anti-Histone H3K27ac (Active Motif Cat# 39134, RRID:AB_ 2722569); anti-NPM1 (Abcam Cat# ab10530, RRID:AB_ 297271; 1:200); anti-Fibrillarin (Abcam Cat# ab5821, RRID:AB 2105785; 1:200); anti-LaminB1 (Abcam Cat# ab16048, RRID:AB_10107828; 1:1000); For imaging studies, all antibodies were diluted in blocking solution. ..

Article Title: RNA promotes the formation of spatial compartments in the nucleus.
Article Snippet: .. Primary antibodies used in the study: anti-Nucleolin (Abcam Cat# ab22758, RRID:AB_776878, 1:500); anti-NPAT (Abcam Cat# ab70595, RRID:AB_1269585, 1:100); anti-SMN (BD Biosciences Cat# 610646, RRID:AB_397973, 1:100); anti-HP1b (Active Motif Cat# 39979, RRID:AB_2793416, 1:200); anti-Coilin (Abcam Cat# ab210785; Santa Cruz Biotechnology Cat# sc-55594, RRID:AB_1121780; Santa Cruz Biotechnology Cat# sc-56298, RRID:AB_1121778; 1:100); anti-Sharp (Bethyl Cat# A301-119A, RRID:AB_873132, 1:200); anti-Histone H3K27ac (Active Motif Cat# 39134, RRID:AB_2722569); anti-NPM1 (Abcam Cat# ab10530, RRID:AB_297271; 1:200); anti-Fibrillarin (Abcam Cat# ab5821, RRID:AB_2105785; 1:200); anti-LaminB1 (Abcam Cat# ab16048, RRID:AB_10107828; 1:1000); For imaging studies, all antibodies were diluted in blocking solution. ..



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